Bioanalytical LC-MS: Streamlined sample preparation and novel ambient ionization techniques

Medicilon offers comprehensive and FDA/CFDA GLP-compliant bioanalysis services to support preclinical and clinical development for small molecule drugs, biologics, vaccines and biomarkers.

Website: www.medicilon.com Tel: +86 (21) 5859-1500

The roles bioanalysis study and bioanalytical study techniques are indispensable in scientific research. In current times, essentially any research in fields dealing with biological sciences, biomolecular systems, or drug pharmacology/toxicology must heavily rely on bioanalysis in order to obtain meaningful results from their experimental data. Researchers in these fields rely on accurate and sensitive bioanalysis to report their findings with statistical confidence. Because of this critical role, a great amount of research efforts have been devoted towards the development of bioanalytical techniques that are better equipped to handle the challenges that the scientific community is currently facing. At times, scientific progress have been hindered due to limitations from an analytical perspective. This ultimately drives the analytical community to find newer, faster, and more versatile solutions to overcome these problems.

Through the years, development of new more powerful analytical methods has been approached by a number of different strategies. For instance, some developments have focused on improving the analytical work with innovations in chromatography or instrumentation [references]. Data analysis is also an emerging area of development where new data processing software and or mathematical treatments of data are introduced to reduce the amount of time required to process large quantities of compiled data [references]. Another area focuses on enhancing sample preparation to increase efficiency towards a high-throughput analytical workflow [references]. Regardless of the approach, innovations in bioanalysis studies generally seek to improve on previous methods from the standpoint enhanced accuracy, sensitivity, and efficiency.

The focus of this dissertation centers are exploring new strategies and approaches to streamline sample preparation towards the direction of fully automated sample preparation workflow. At times, sample preparation can represent the majority of consumed resources and required labor causing a significant detriment to method efficiency. In addition, many of the common sample preparation techniques are somewhat lacking in terms of their selectivity and recovery leading to issues with sample loss. Clearly, there must be an effort to seek better, more efficient methods of sample preparation. Two potential solutions to challenges faced with sample preparation are investigated for their potential application in future research. First, the use of restricted access media (RAM) is explored through the form off different liquid chromatography trap technologies. The second approach uses ambient ionization, or atmospheric pressure ionization, techniques are a means another means to circumvent sample preparation entirely. This first section of the dissertation will cover a general overview of the topics to be discussed later in the remainder of the literary work.